Question
Size-Exclusion (Gel Exclusion) Chromatography
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Answer
Separates by size; MCAT clue: large molecules elute first.
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Question
SDS-PAGE
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Answer
Separates by size; MCAT clue: smaller molecules run farther.
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Question
Isoelectric focusing
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Answer
Separates by charge/pI; MCAT clue: proteins focus where pH equals pI.
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Question
Affinity chromatography
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Answer
Separates by specific binding; MCAT clue: target binds a specific ligand.
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