Question
Disulfide bond formation vs reduction: which redox process forms the S−S bond, and what do DTT/BME do?
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Answer
Formation is oxidation; DTT/BME reduce and break S−S bonds.
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Question
What catalyzes peptide bond formation in cells, and what energy source is consumed?
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Answer
The ribosome's rRNA catalyzes it, consuming GTP.
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Question
Why are polypeptides written from N-terminus to C-terminus?
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Answer
By convention, and translation adds residues to the C-terminus.
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Question
A linear polypeptide has 150 residues. How many peptide bonds and water molecules were produced?
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Answer
149 of each; linear chains have n−1 bonds and release n−1 waters.
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