Question
If that unknown protein is about 40kDa, does it run above or below the 25kDa band?
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Answer
Above; it is larger, so it migrates less far.
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Question
Why do more volatile compounds elute first in GLC?
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Answer
They stay more in the gas phase and partition weakly into the liquid film.
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Question
Retention factor (Rf)
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Answer
Spot distance divided by solvent-front distance; planar chromatography clue: 0 to 1.
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Question
Why do large molecules elute first in size-exclusion chromatography?
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Answer
They are excluded from pores and take the shortest path around the beads.
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