Question
Quaternary structure vs monomeric protein
Tap to flip
Answer
Quaternary structure requires multiple polypeptide subunits; a monomer has none, even if it crystallizes as a dimer.
Tap to flip back
Question
Why is Vmax described as a theoretical limit rather than an observed maximum?
Tap to flip
Answer
It is approached asymptotically as all enzyme becomes saturated.
Tap to flip back
Question
Why is hydrogen bond formation alone not the main driving force for protein folding?
Tap to flip
Answer
Similar numbers of H-bonds exist in folded protein and unfolded protein plus water.
Tap to flip back
Question
What did Anfinsen's RNase A experiment show about protein structure?
Tap to flip
Answer
Primary sequence alone determines native 3D structure under physiological conditions.
Tap to flip back