Question
Why does a protein move toward the anode in IEF when local pH>pI?
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Answer
Above its pI it is net negative, so it moves to +.
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Question
Theoretical plate
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Answer
One effective vaporization-condensation cycle in a fractionating column.
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Question
exclusion limit
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Answer
Size at or above which molecules enter no pores and elute together unseparated.
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Question
Ion-exchange chromatography (IEX)
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Answer
Separation by net charge on a charged resin; clue: use pH vs pI to predict binding.
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